Description
GenJet� DNAIn VitroTransfection Reagentfor epithelial cell is pre-optimized for epithelialcells. By cross-linking a peptide ligand, this pre-optimizedGenJet� reagent specifically binds epithelial cellsand give superior efficiency on epithelial cells.
Refer to the following optimal transfection conditions for maximal transfection efficiency on epithelial cells. GenJet� reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet� (�l) : DNA (�g) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet�/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours
EGFP
pEGFP-N3 (CMV promoter)
65%
|
Storage Condition
Store at 4 �C. If stored properly, the product is stable for 12 months or longer
A comparison showing superior efficiency of the pre-optimized GenJet (Ver. II) on normal human colonic epithelialcells.Transfection was performed per the vendors' protocol. 0.5�g of GFP DNA (pEGFP-N3) per well in 24-well plate was delivered to normal human colonic epithelial cells with different transfection methods. The GFP positive cells were counted under a Nikon 2000 Eclipse fluorescence microscope 24 hours post transfection.
Data Sheet
