Application:
用于重新探测和质谱的枝条印迹
Features:
- Strip blot in 5~15 minutes
- 在室温下孵育
- No stench smelling without addition of 2-mercaptoethanol or its analogs
- A propriety formulation with an eco-friendly and non-hazardous organic solvent (with light odor) included to completely disassociate the bound antibodies
Application I. Strip Western Blot for Re-probing
A Comparison of BlotFresh� Stripping Reagent with Pierce's Restore� Blot Stripping Buffer
- Blot Stripped and Re-probed with the Same Antibody*:
Total Akt (catalog # A0501-1) with different treatments in NIH 3T3 cells
* Develop 2 minutes with ECL Plus�and expose 20 seconds on Kodak negatives
- BlotFresh� Stripping Reagent is More Effective in Breaking Down the Antigen-Antibody Complex than Pierce's Restore� Stripping Buffer:
BlotFresh�剥离Reagent*Pierce的恢复剥离缓冲器*
原版的Expose x 1'Expose x 5'原版的Expose x 1'Expose x 5'
* The blots were initially probed with β-actin antibody with exposure for 10 seconds. Then the blots were stripped with BlotFresh� Stripping Reagent for 5 minutes and with Pierce's Restore� Stripping Buffer for 15 minutes respectively. After washes, the stripping effect was tested by development of blots with ECL Plus� for 2 minutes followed by exposure to Kodak BioMax films for 1 and 5 minutes respectively
- BlotFresh�Stripping Reagent Strips aBlot followed by Re-probing with a Different Antibody:
A.用抗体探测全部AKT的抗体（目录＃A0501-1）*。
B.Blot was stripped with BlotFresh� Stripping Reagent and re-probe with antibody against phospho-Akt (catalog # A0503-1)*.
*泳道1是血清饥饿过夜，而在NIH3T3细胞中以不同浓度的PDGF处理泳道2〜5。The blots were developed 2 minutes with ECL Plus�试剂并在柯达底片上暴露10秒（ECL Plus�is a trademark of GE Healthcare-Amersham)
Application II. Strip Western Blot for Mass Spectrometry
Procedures for Stripping Western Blot for Mass Spectrometry:
- Strip blot for 15 minutes at RT to remove primary and secondary antibodies
- Locate the protein by comparing the stripped blot and exposed film and cut the band from blot
- 消化切割带，然后进行质谱（参见下图）
Data Sheet
Testimonials:
...接受了污黑的蛋白质印迹溶出试剂样品。梦幻般的产品!!已经习惯于剥离相同的印迹2x，并产生三种射线照片与第二两种抗体的主要墨水无法区分。我今天会订购一些......
------凯瑟琳博士，NCI，NIH
I cannot live without it! It is just amazing. I used to use Pierce�s and I often need to re-run Western due to dirty blot after strip. Since I started using this product, my life has been changed� It makes my research easier and I do not need to re-run gel anymore� And the stripping process is just 2 minutes. It is really amazing product!
------by Dr. Jennifer Lin, the George Washington University
一些产品引文：
1. Mahmoud M. El-Mas, Ming Fan, and Abdel A. Abdel-Rahman. Endotoxemia-Mediated Induction of Cardiac Inducible Nitric-Oxide Synthase Expression Accounts for the Hypotensive Effect of Ethanol in Female Rats.J. Pharmacol。Exp。它。2008; 324: 368-375
2. Grzegorz Woszczek, Li-Yuan Chen, Sahrudaya Nagineni, Sara Alsaaty, Anya Harry, Carolea Logun, Rafal Pawliczak, and James H. Shelhamer. IFN-gamma Induces Cysteinyl Leukotriene Receptor 2 Expression and Enhances the Responsiveness of Human Endothelial Cells to Cysteinyl Leukotrienes.The Journal of Immunology, 2007; 178: 5262-5270
3. David A Baker，Aric Madayag，Lars V Kristianse，James H Meador-Woodruf，Vahram Haroutunia和Ilangovan Raju.Contribution of Cystine�Glutamate Antiporters to the Psychotomimetic Effects of Phencyclidine.Neuropsychopharmacology, 2007; Aug. 29 online publication
4. Ashley B. Williams and Patricia L. Foster. The Escherichia coli Histone-like Protein HU Has a Role in Stationary Phase Adaptive Mutation.Genetics.2007; 177:723�735
