LipoD293� DNA In Vitro Transfection Reagent
Description
By utilizing our innovative and proprietary lipid conjugation technology, LipoD293� (Ver. II) is specially designed and formulated by adding proprietary enhancers for transfecting HEK293 cells and other mammalian cells (Figure 1). As a 2nd generation liposome based DNA transfection reagent, LipoD293� (Ver. II) offers extremely high transfection efficiencies for HEK293 related cells as well as many mammalian cells with less cytotoxicity. LipoD293� reagent (Ver. II), upgraded from its previous version with refined chemistry, is 3~4 times more efficient in gene delivery. LipoD293� reagent (Ver. II), 1.0 ml, is sufficient for ~666 transfections in 24 well plates or ~333 transfections in 6 well plates.
Figure 1.A Cartoon Showing LipoD293� (Ver. II) Enhanced Gene Delivery
特征
- Top choice for hard-to-transfect cells
- Exceptional high titers of virus production
- Equally good for very long DNAs (up to 180 kb)
- Equally good for suspension 293 cells (e.g., 293F, 293H, etc)
- High levels of recombinant protein production
- Presence of serum and antibiotics enhances efficiency on 293 cells
- Exceptional efficiency for both single DNA transfection and multi DNAs co-transfection
- 非常负担得起
Storage Condition
Store at 4 �C. If stored properly, the product is stable for 12 months or longer.
LIPOD293的转染效率的比较在体外转染试剂（VER。II）与品牌产品的比较
Comparison of transfection efficiency of LipoD293� reagent (Ver. II) vs. lipofectamine 2000 (L2K) and Fugene HD on HepG2 cells.
Right Panel: Comparison of transfection efficiency of LipoD293 (Ver. II) with Lipofecatmine 2000 (L2K), and Fugene HD on HepG2 cells. GFP DNA (pEGFP-N3) was transfected with different transfection reagents per manufacturer's protocols to HepG2 cell (cultured on Collagen pretreated dishes). GFP positive cell (%) and fluorescence intensity were detected by passing through FACS 48 hours post transfection
左面板：presence of serum and antibiotics enhances LipoD293 (Ver. II) efficiency on HepG2 cells. HepG2 cell (grown on collagen treated dishes) was transfected with three different conditions-------serum and antibiotics free, presence of 10% serum and antibiotics followed by removal 5 hours post transfection and presence of 10% serum and antibiotics without removal 5 hours post transfection.
LIPOD293试剂的转染效率（VER。II）与Lipofectamine 2000（L2K），Transit和Fugene 6上的转染效率的比较。
Right Panel:Comparison of transfection efficiency of LipoD293 (Ver. II) with Lipofecatmine 2000 (L2K), TransIT and Fugene 6 on CHO cells. DNAs encoding Renilla luciferase (phRL-CMV) and GFP (pEGFP-N3) were transfected with different DNA transfection reagent per manufacturer's protocols. Renilla luciferase activity and GFP fluorescence were detected with Renilla Assay System and a Nikon Eclipse fluorescent microcopy respectively 24 hours post transfection.
左面板：Comparison of price ($/1.0 ml vial) of LipoD293 versus those of Lipofecatmine 2000 (L2K), TransIT and Fugene 6. All the prices were collected from the manufacturers' websites.
A comparison of transfection efficiency of LipoD293� reagent with lipofectamine 2000 (L2K) on a hard-to-transfect cell, primary rat aortic smooth muscle cells.The rat aortic smooth muscle cells were prepared and transfected with pEGFP-N3 by LipoD293� reagent (left panel) and Lipofecatmine 2000 (L2K, right panel) respectively per manufacturers' protocols. The transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon Eclipse 2000 microscopy 24 hours post transfection. The above pictures were kindly provided by Dr. Nickolai Dulin of Section of Pulmonary and Critical Care, University of Chicago.
A comparison of transfection efficiency of LipoD293� reagent with Fugene HD on hard-to-transfect cell, LNCap cells.The LNCap cells were grown as ATCC recommended procedures and co-transfected with pBabe-hygro-SSeCKs (1.5ug) and pEGFP-N3 (0.5ug) per well (6 well plate) LipoD293� reagent (left panel) and Fugene HD (right panel) respectively per manufacturers' protocols. The transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon Eclipse 2000 microscopy 24 hours post transfection. The above pictures were kindly provided by Dr. Lyn Gao of Roswell Park Cancer Institute.
Two examples showing exceptional efficiency of LipoD293� reagent on hard-to-transfect cells like HepG2 and SaoS-2 cells.HepG2 and SaoS-2 cells in 95% confluency were transfected with pEGFP-N3 and pSV-?-galactosidase DNAs respectively in presence of serum/antibiotics. The efficiency was checked 48 hours post transfection by Zeiss 510 Confocal Microscopy and ?-galactosidase staining kit respectively.
A comparison of transfection efficiency of LipoD293� reagent with 293fectin on HEK293 cells.HEK293细胞使用LIPOD293。在体外DNA转染试剂（VER。II）（上图）和最流行的Invitrogen（下面板）最受欢迎的品牌产品293fectin。通过DIC相成像（左图）和FITC成像（右图）24小时转染后24小时，通过Nikon Eclipse荧光显微镜观察细胞。
A comparison of LipoD293� reagent vs. 293fectin, Xfect and Fugene 6 transfection reagents on protein production with suspension 293F cells. 30 mL of293F cell cultured in standard culture medium was transfected with pEGFP-6xHis plasmid using LipoD293� In Vitro DNA Transfection Reagent (Ver. II) (20 ug plasmid DNA), 293Fectin (30 ug plasmid DNA), Xfect (30 ug plasmid DNA) and Fugene 6 (30 ug plasmid DNA) per manufacturers' standard transfection protocols. GFP fluorescence was visualized 48 hours post transfection (left panel) with A for LipoD293, B for 293fectin, C for Xfect and D for Fugene 6. The 6xHis tagged GFP protein was then purified vini-ntaaffinity column. 5 ul of 1st elution fraction was resolved on SDS-PAGE followed by Coomassie Brilliant Blue staining (right upper panel E) with the lane 1 for LipoD293, lane 2 for protein marker, lane 3 for Xfect, lane 4 for 293fectin and lane 5 for Fugene 6. The protein yield was quantified via spectrometer (right lower panel F).
A comparison of LipoD293� (Ver. II) and Lipofecatmine LTX (LTX) on generation of Lentivirus (LV).三个互补与LipoD293 co-transfected�(Ver. II) and Lipofectamine LTX (LTX) into 293FT cells. A GFP vector, pHR-SIN-cppt-CMVEWP, was used to determine titer of LV. 1x105 293F cells per well was plated in to a 24 well plate followed by addition of different of amounts of the vector supernatant, 1 microliter (upper panel) and 10 microliters (lower panel) respectively. 5 days later, the cells was passed with FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of LV generated with LipoD293� and L2K were quantified to be 8x10^6 and 3x10^6 tu/ml respectively
Technical Information & Datasheet
-Lipod293。（VER。II）试剂一般协议和数据表
-LipoD293� (Ver. II) Reagent Short Protocol
-LipoD293� (Ver. II) Reagent Protocol for Transfecting Suspension 293 and CHO Cells
-LipoD293� (Ver. II) Reagent for Lentivirus Production
-LipoD293� (Ver. II) Reagent for rAAV Production
-Technical Note & Transfection Tips
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Testimonials
Lipod293转染试剂感到非常兴奋！我将LipoD293与脂肪系胺进行了比较，以转染质粒，以产生病毒式颗粒和哇！我使用LipoD293试剂比Lipofectamine恢复了4倍的病毒！！这对我和我的导师来说是个好消息，因为这意味着我不必花太多时间（和资源）用于转染293T细胞来恢复体外实验的病毒。我的导师也很高兴Lipod293的成本比Lipofectamine低得多！我们已经订购了5毫升的lipod293，现在我说服了我的实验室伴侣切换到Lipod293，因为我对此取得了如此出色的效果。感谢您制作如此出色的产品！
-------- Christy Lavine, Ph.D., Harvard University
我想在您发送给我们的LipoD293的免费样品中向您更新。我最近与Lipofectamine 2000并排转染了一些Plat-E的并排，您的产品超越了它！！！我们还使用HeLa细胞来验证它，但否则我们对迄今为止所看到的东西感到非常满意！感谢您向我们发送免费样本，它一定会为我们销售！
--------Catherine Gallo, Ph.D., University of Cincinnati
we have now tried the LipoD293 DNA In Vitro transfection reagent on 293FT cells. In the first flask we used the protocol by Invitrogen provided in theVirapower box insert (Virapower, pBABE-GFP plasmid, plus Lipofectamine). In the second flask we used LipoD293 (30 ul/6ml media) with the same amounts of Virapower and plasmid as in the first flask. The results were stunning:LipoD293 gave twice as many transformants as Lipofectamine 2000...
-------- A Beta Tester from Wayne State University
