rAAV Production Full Service, Pilot Scale

Features:
- Nonpathogenic with least immune response.
-Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
- Persistent transgene expression.
- Multiple serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ).

Large AAV cis vector inventory for constructing AAV cis plasmid with a specific promoter and reporter:

Service Description:
- Cloning your gene of interest (GOI) into AAVcisvector.
- rAAVcisplasmid large prep.
- rAAV packagingin 10x150 mm plate.
- rAAV purification via advanced 2xCsCl ultra-centrifugation.
-Desalting, filter sterilization and AAV titration via qPCR.

rAAV Serotypes We Offer:
AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, AAV-DJ/8 and AAV-DJ.

Required Materials:A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).

Turnaround Time:2 ~ 3 weeks.

Deliverables:>0.1 mL (2x50µL) of super purified in vivo grade rAAV vector at >1E+13 VG/mL*.

We offer discount for new customer, pleaserequest a quotewith us today.

Super High Yield:
With our proprietary genetically engineered AAV·HT™ packaging cell and a advanced purification approach, the rAAV yield is easy to reach super high level--------total 1E+15 VG. For super high level rAAV production up to 1E+15 VG total, pleasecontact usto request a quote.

Figure 1. A comparison of purity and infectivity of rAAV vectors from different sources showing super purified and super infectious (close to clinical trial grade) rAAV vector prepared via our advanced double CsCl ultra-centrifugation approach.
A. rAAV vectors (total 1E+9 VG per lane) from different sources were resolved on SDS-PAGE followed by silver staining. Lane 1: GMP manufactured rAAV vector from CHOP; Lane 2: rAAV prepared via our advanced 2xCsCl ultra-centrifugation approach; Lane 3: rAAV from Vector Core of BCM; Lane 4: rAAV from our competitor "V"; Lane 5: rAAV from our competitor "C"; Lane 6: Protein marker.
B. Super infectious rAAV vector prepared via advanceddouble CsCl ultra-centrifugation. Left panel: rAAV2-CAG-GFP (total 5E+9 VG) from our competitor "V" injected to mouse eye; Right panel: rAAV2-CAG-GFP (total 5E+9 VG) purified via advanced 2xCsClultra-centrifugationinjected to mouse eye.


Figure 2. A comparison of infectivity of rAAV vectors from different sources showing super purified rAAV prepared via advanced double CsCl ultra-centrifugation approach is super infectious.
rAAV1-GFP (total 2E+9 VG) from different sources were injected to mouse muscle tissue. The GFP fluorescence was visualized 3 weeks post injection. A. rAAV1-GFP from our competitor "V"; B. rAAV1-GFP from our pre-made rAAVs stock purified via advanceddouble CsCl ultra-centrifugation. C.Quantification data showed that our super purified rAAV (bar 2) is ~ 9 times more infectious than that (bar 1) prepared via conventional CsCl ultra-centrifugation.

* Final viral yield may depend on the nature of transgene.