Pre-made recombinant AAV; AAV-CAG-FLPo; AAV-CBA-FLPo; AAV9-CAG-FLPo; AAV9-CBA-FLPo
Product Category:Pre-made recombinant AAV; AAV-CAG-FLPo; AAV-CBA-FLPo; AAV9-CAG-FLPo; AAV9-CBA-FLPo
Description:FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 �C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse.This AAV9-CAG-FLPopre-made rAAV in serotype 9 willexpress FLPo recombinase underCAG promoter (also known as CBA promoter).Ready to use format.
Titer:> 1x1013VG/mL
Size:>30�L (Std Pack)
Storage Buffer:PBS
Infection Protocol:
Vector Map: