Pre-made recombinant lentivirus; LV-EF1α-SpCas9; LV-EF1α-Cas9; LV-EF1A-SpCas9; LV-EF1A-Cas9; EF1A-Cas9 Lentivirus
Product Category:Pre-made recombinant lentivirus; LV-EF1α-SpCas9; LV-EF1α-Cas9; LV-EF1A-SpCas9; LV-EF1A-Cas9; EF1A-Cas9 Lentivirus
Description:LV-EF1α-SpCas9is a pre-made lentivirus which over-expressesSpCas9under the EF1αpromoterandcan be used along with guided RNA for genome-editing. The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. Cas9 (CRISPR associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes' CRISPR immunity system. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.Ready to use format.
Titer:> 1x109TU/mL
Selection Antibiotic:None
Purity*:In vivo grade and OK for both in vitro tissue culture infection and in vivo injection
Size:2 x 25�L (Std Pack)
Storage Buffer:PBS
Infection Protocol:
Transfer Vector Map:
*Concentrated viaPEGprecipitation followed by ultra-centrifugation at 25000 rpm
