Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-CBA-FLPo; AAV6-CMV-FLPo; AAV6-CBA-FLPo
Product Category:Pre-made recombinant AAV; AAV-CMV-FLPo; AAV-FLPo; AAV6-CMV-FLPo; AAV6-FLPo
Description:FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the φ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 �C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse.This AAV6-CMV-FLPo pre-packaged rAAV in serotype 6 (with capsid from AAV serotype 6 and 2xITR from AAV serotype 2) willover-express FLPo recombinase underCMV promoter.Ready to use format.
Titer:> 1x10^13 VG/mL
Size:>30�L (Std Pack)
Storage Buffer:Sterile PBS
Infection Protocol:
Vector Map:
