genmutesiRNA Transfection Reagent

Description:
genmuteReagent is a novel biodegradable non-liposomal siRNA/miRNA & DNA delivery tool. With our proprietarypH Dependent Conformational Change (PDCC技术，可生物降解的转染试剂是通过在其侧链中添加预筛选的疏水组来制定的，从而使genmuteReagenta versatile and most powerful gene delivery tool in the market.genmuteReagenthave been validated to effectively and reproducibly transfect single siRNA/miRNA or co-transfect DNA/siRNA or DNA/miRNA to variety of mammalian cells.

Size & content:
- Genmute-试剂，1.0毫升，基于24孔板中的5.0 pmol siRNA或miRNA模拟物，足以实现约2,000个反应。
- Genmute�转染缓冲液（5X），用于最大转染效率，8.0 mL，以制造40 mL工作溶液。

应用程序:
- Single siRNA transfection or DNA/siRNA co-transfection to variety of mammalian cells.
- 单miRNA，miRNA模拟或抑制剂转染或DNA/miRNA或DNA/miRNA模拟转染到各种哺乳动物细胞。

Storage:
Store at 4 �C forgenmuteReagent and RT forgenmute转染缓冲液（5x）. If stored properly, the product is stable for 12 months or longer.

Advantages:
- Excellent silencing at low concentrations with only 1.0 nM siRNA / miRNA mimics
- Minimizing non-specific and off target effects in the cell
- One tube reaction
- Best for broad mammalian cells
- Very low cytotoxicity
- 非常负担得起

GenMute的沉默功效的比较 - siRNA和DNA转染试剂与品牌产品产品

Genmute转染试剂（上图）与Dharmafect的敲低功效比较4 siRNA转染试剂（中间面板）and Lipofectamine� 2000 (lower panel) on HEK293 cells.siRNA以不同的最终浓度靶向肾小球荧光素酶，范围为0.5至20 nm，与肾素荧光素酶基因（0.5 g prl-cmv dnA孔）共转染，该基因由以上三种转染试剂每个制造商的方案中的三种转染试剂）与HEK293细胞中的三种转染试剂共染色（24孔板。Renilla luciferase activity was determined 24h after post co-transfection with renilla luciferase determination system (Promega). The luminescence was measured from 5.0 �l of lysate during 10s integration with a luminometer (Beckman Coulter LD 400). Luciferase activity wasexpressed as light units integrated over 10s (RLU) and normalized per mg of cell protein by using the BCA assay. The errors bars represent standard deviation derived from triplicate experiments. Luciferase-silencing efficiency was calculated relative to untreated cells.尽管genmute。pofectamine� 2000 gave good knockdown only after 30 nM (data not shown).


在HEK293细胞中，内源表达的KIF11（也称为EG5）的出色沉默，具有0.5 l genmuTE剂和5.0 pmol EG5 siRNA每孔24孔板。KIF11 (also known as Eg5) encodesa motor protein that belongs to thekinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. GenMute� reagent effectively delivers Eg5 siRNA (final 10 nM, right panel) to HEK293 cells at only 0.5 �l per well of 24-well plate vs. negative control (final 10 nM. left panel). Compared with negative control (left panel), phenotype of "rounded-up" 293 cells were visualized 24 hours post transfection (right panel) with a Nikon microscope.


genmuteTransfection Reagent knocked down endogenous lamin A/C gene expression in Hela cells.A siRNA targeting lamin A/C gene (right panel) and a sham siRNA (left panel) were introduced into Hela cells (final 1.0 nM) bygenmuteTransfection Reagent. Lamin A/C gene silencing was monitored 24h post transfection by immunofluorescence. Lamin A/C was probed with a mouse monoclonal lamin A/C specific antibody followed by addition of FITC-conjugated anti-mouse antibodies. Quantitative analysis showed thatlamin siRNA at 1.0 nM delivered by GenMute� Transfection Reagent knocked down 94% endogenously expressed lamin A/C in Hela cells.

Data Sheet & Protocol
-A Protocol for siRNA Transfection and DNA/siRNA Co-Tranafection
-A Protocol for miRNA Mimics Transfection and DNA/miRNA Co-Transfection
-A Protocol for DNA Transfection to Mammalian Cells

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