PolyJet� In Vitro DNA Transfection Reagent

Description
Based on our proprietary polymer synthesis technology, PolyJet� DNA In Vitro Transfection Reagent is formulated as a biodegradablepolymer基于DNA转染试剂，可确保对HEK293的有效和可重复转染，COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian celLS。PolyJet�试剂能够以非常低的浓度在电泳过程中固定DNA迁移，并在RT的5分钟内形成转染复合物。该试剂的一个显着特征是转染复合物内吞作用后聚合物的快速和完全降解（图1），导致细胞毒性少得多。Polyjet r试剂（1.0 mL）足以在24个井板中进行〜667次转染或6个井板中的〜333转染，为领先产品提供了一种非常实惠的替代品，用于转染各种常用和难以转移的哺乳动物细胞。


Figure 1。一部动画片，显示了聚会的生物降解�DNA Transfection Reagent After Endocytosis of Transfection Complex

Features
- Bio-degradable after endocytosis
- Exceptional high titers of virus production
- Equally good for very long DNAs (>89 kb)
- Equally good for both single DNA transfection and multi DNA co-transfection
- High levels of recombinant protein production
- Simple & robust transfection procedure
- 非常负担得起

Storage Condition
Store at 4 �C. If stored properly, the product is stable for 12 months or longer.

哺乳动物细胞类型的广泛转染光谱

Examples Showing Transfection Efficiency of PolyJet� DNA In Vitro Transfection Reagent on Commonly Used Cells

Transfection efficiency comparison of PolyJet� vs. lipofectamine Plus on Chinese Hamster Ovary (CHO) cells.HA tagged beta-tubulin cDNA was delivered into CHO cells with PolyJet� (left panel) and lipofectamine Plus (right panel) respectively. FITC conjugated antibody against HA tag was utilized to pick up HA-beta-tubulin (Green) while a DM1a antibody was used to detect endogenous alpha-tubulin followed by probing with rhodamine conjugated secondary antibody (Red). The above picture was provided by Dr. Shang Yin of University of Texas at Houston Medical School as courtesy


A comparison showing transfection efficiency of PolyJet� reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells.HEK-293FT cells were transfected with GFP vector (pEGFP-N3) by PolyJet� (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection


A comparison showing transfection efficiency of PolyJet� reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells.HepG2细胞转染GFP向量(pEGFP-N3) by PolyJet� (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection


Transfection efficiency comparison of PolyJet� vs. Fugene HD on MDCK cells.A plain GFP DNA was transduced into MDCK cells with PolyJet�(left panel) and Fugene HD (right panel) reagents respectively per manufacturers' protocols. GFP and DAPI staining were visualized under fluorescence microscopy 48 hours post tansfection. The above comparison data and pictures were completed and provided by Dr. Ge Zhou of NYU Medical Center as courtesy


A comparison showing transfection efficiency of PolyJet� reagent vs. a leading product, Lipofectamine 2000 on MDCK cells.MDCK cells are notoriously hard to transfect. With proprietary "Shaved Cell Transfection" protocol, PolyJet� (left panel) gives up to 70% GFP positive cells vs. Lipofectamine 2000 (right panel) around 5% efficiency. MDCK cells were transfected with GFP vector (pEGFP-N3) by PolyJet� (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 36 hours post transfection


A comparison showing transfection efficiency of PolyJet� reagent vs. a leading product, Fugene HD on LNCap cells.LNCap cells were transfected with GFP vector (pEGFP-N3) by PolyJet� (left panel) and Fugene HD (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection


我mage showing exceptional transfection efficiency of PolyJet� reagent onHuman embryonic stem cells(hESCs).ThehESCgrown in E8 medium on Geltrexvs (left panel, DIC imaging) was transfected with pEF1α-GFP.The GFP expression (right panel) was visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.The above pictures were provided byDr.Marina Pryzhkovaof Johns Hopkins University as courtesy


Neuro2A cells transfected with pEGFP-C1 plasmid using PolyJet�In VitroDNA Transfection Reagent.The Neuro2A cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left) and FITC imaging (right) 24 hours post-transfection


Comparison of cytotoxicity of PolyJet�DNA In Vitro Transfection Reagent with L2K� on primary murine skin fibroblast.The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection

DATA表和协议
-General Protocol for Transfecting Mammalian Cells
-A Short Protocol for Transfecting Mammalian Cells
-用于转染难以转化的哺乳动物细胞的高级方案
-A Protocol for Transfecting Suspension 293 and CHO Cells
-Protocol for Lentivirus Production
-Protocol for rAAV Production
-Technical Note & Transfection Tips


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To request a free trial sample, pleaseCreate An Accountwith us to enter your shipping address and email us atorder@www.gjp158.com




Testimonials:

Sorry for sooo big delay, but now I am totally in love with PolyJet. I have used PolyJet for a while on mouse ESCs with relatively good efficiency 50~70%.

--------Marina Pryzhkova, Ph.D., JHU

PolyJet Transfection Reagent worked equally as well as lipofectamine 2000, with little evidence of cell death on 293, PC-3 and 22RV1 cells. I will defiantly consider switching over.

-----蒂法尼·华莱士（Tiffany Wallace）博士，NCI / NIH

我只与测试样品（PolyJet）和LiPO2000并排进行了cos-7细胞的GFP转染。结果非常好。Polyjet甚至比L2K更好。

------Feng Qiao, Ph.D., NEI / NIH

I tested the sample of PolyJet on my NIH-3T3 mouse fibroblasts this weekend. The results were much better than Lipofecatmine LTX. I'm attaching a powerpoint slide with my results (I did not quantify the % transfection efficiency, but the pictures get the point across). I found that the protocol for difficult-to-transfect cell lines worked better than the standard protocol.

-----斯蒂芬妮·墨菲（Stephanie Murphy）博士，达特茅斯学院

I had chance to try your product finally. It was great success. I used HeLa cells and got 10% transfection efficiency (<0.1% for Lipofectamine). Thank you! I was wondering if I also try GenJet� Plus DNA In Vitro Transfection Reagent? According to your website, the reagent works better than regular PolyJet.

-------Yumi Uetake, Ph.D., UMASS

I tested PolyJet and it looks great on MDCK. We placed order. Thank you!

-------Ge Zhou, Ph.D., NYU

We are happy to provide feedback. PolyJet worked very well for us in HepG2 cells, we got approximately 80% efficiency with pMAX GFP plasmid, by following the conditions in your suggested protocol. We ran a comparison with Lipofectamine, which only showed approximately 20-30% transfection efficiency. We are planning experiments and will be ordering more soon.

-------Emily Mcallister, PBRC