LipoJet�体外转染工具包(版本。II)

Description:
Based on our innovative and proprietary lipid-conjugation technology, LipoJet™ Transfection Kit, formulated fromnovel fluorinated cationic lipids, exhibits significant advantages from oth市场上的ER脂质转染试剂。LipoJet™转染套件是用于多种哺乳动物细胞类型的各种应用，包括质粒DNA和/或siRNA，包括质粒DNA和/或siRNA，是最强大但非常温和的基因输送工具。Compared with leading products in the market,LipoJet™ is morecost-effective and always provides higher transfection efficiency with less cytotoxicity.

套件内容：
- LipoJet™ Reagent, 1.0 ml sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate).
- LipoJet™ Transfection Buffer (5x ), 8.0 mL to make 40 mL of working solution (1x ).

Features:
- Versatile: DNA transfection, siRNA transfection and DNA/siRNA co-transfection.
- Powerful:Outstanding efficiency on adherent cells.
- 极低的毒性：转染后提高细胞活力
- Economical: More transfections with less reagent
- Easy to use: One tube reaction and compatible with serum/antibiotics.
- Suitable for high-throughput application

Storage:
Store at 4 �C forLipoJet� Reagent and RT for LipoJet� Transfection Buffer (5x ). If stored properly, the product is stable for 12 months or longer.

哺乳动物细胞类型的广泛转染光谱

Cell Line	DNA Transfection	siRNA Transfection
293, 293T 3T3, NIH 3T3 3T3-L1 A549 B16-F10 BNLCL.2 C2C12 C3H/10T1/2 Caco-2 Rat primary cardiomyocytes CHO COS1 COS7 人膀​​胱癌细胞 mes iMEF HBEC HCT116 Hela HepG2 HT-29 HuH-7 LNCaP MC3T3-E1 MCF10A MCF7 MDA-MB-231 MDCK MEF Human primary melanocytes Human primary melanoma Human immortalized microglial mIMCD-3 MRC-5 N2A PC3 RAW 264.7 RPE SH-SY-5Y SK-OV-3 U-2OS VERO hESC	85% 65% 30% 80% 75% 55% 80% 60% 20% 10％ 80% 70% 75% 80% 50% 55% 45％ 75% 80% 60% 45％ 45％ 45％ 50% 40% 50% 50% 20% 50% 50% 50% 50% 50% 50% 68% 70% 45％ 30% 75% 70% 80% 40% 70%	- 90% at 20 nM - 90% at 10 nM - - 90% at 10 nM - 80 % at 20 nM - - - - - - - - - - - - - - - - - - - 30 nm时85％ 30 nm时85％ - - - - - - - - 90% at 25 nM - - - 90% at 40 nM

Examples Showing Excellent DNA and siRNA Transfection Efficiency of LipoJet™ In Vitro Transfection Kit

A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO and HEK293 (left panel) and Cos-7 (right panel).pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers. The transfection efficiency were analyzed via FACS 48 hours post transfection.


A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells.pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers. The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection.


A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell.PEGFP-N3 cDNA（0.125 µg/孔，0.25 µg/孔和24孔板的每孔0.5 µg/每孔）使用标准转染方案转染到293T细胞中，并在具有LipoJet™（lipojet™）的情况下（10％FBS）（上）Lipojet的面板™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA(µl/µg)ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA(µl/µg)比率= 3）。转染后48小时，通过尼康日食荧光显微镜可视化细胞。


A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell.pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA(µl/µg)ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA(µl/µg)比率= 3）。转染后48小时，通过尼康日食荧光显微镜可视化细胞。




Exceptional gene silencing of LipoJet™ reagent during DNA/siRNA co-transfection on mammalian cells.转染GFP cDNA（左面板，每孔24孔板0.25 µg）和GFP cDNA（每孔24孔板0.25 µg）和GFP靶向siRNA（最终10 nm，右图）的共转染（0.25 µg）™试剂分别导致HEK293（上图），HELA（中图）和SAOS-2（下图）细胞的特殊基因沉默。转染后48小时，通过尼康日食荧光显微镜可视化细胞。


Exceptional DNA/siRNA co-transfection efficiency on HUVEC.用LipoJet™试剂共染色（每孔24孔板0.10 µg）和FITC共轭siRNA（最终30 nm孔24孔板）到HUVEC，从而增加了60％MCHERRY+（与相比倍增，与相反转染后24小时，MCHERRY成像，左图）和近100％FITC-SIRNA+（相对鲜明对比与FITC成像，右图重叠）HUVEC。这些图片是从南加州大学的潘·基博士（Pan Kong）提供的，作为礼貌。



用脂肪杰特的HEK293（上图）和Hek293（上图）和HELA（下图）中的内源表达的KIF11（也称为EG5）的出色沉默™ reagent at 10 nM EG5 siRNA.KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.


A image showing exceptional transfection efficiency of LipoJet� Transfection Kit上Human embryonic stem cells(hESCs).ThehESCgrown in E8 medium on Geltrexvs (left panel, DIC imaging) was transfected with pEF1α-GFP.The GFP expression (right panel) was visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.以上图片由Dr.Marina Pryzhkova约翰·霍普金斯大学（Johns Hopkins University）的礼貌

数据表和协议：
-A Protocol for DNA and siRNA Transfection
-A Short Protocol for DNA Transfection
-A Short Protocol for siRNA Transfection
-DNA /核协议Co-transfection
-Technical Note & Transfection Tips

We are offering "Wireless PowerPoint Presenter" to customers who purchase 5x1.0 mL LipoJet™ reagent by December 2014. Take advantage of it and don't let this chance go...

FG-3: Wireless PowerPoint Presenter with Case for purchase of 5 X 1.0 ml or more. This presenter is offered for limited time only!

To request a free trial sample, pleaseCreate An Accountwith us to enter your shipping address and email us atorder@www.gjp158.com




Testimonials:

Sorry for sooo big delay, but now I am totally in love with both PolyJet and LipoJet.Recently we discovered that LipoJet has super good transfection efficiency with mESCs and hESCs withi much less toxicity. Last week I transfected overnight human ESC line H1 using LipoJet with same amazingly high efficiency - up to 50~70%. DNA construct includes GFP under EF1a promoter (CMV promoter does not work in hESCs). This is the best transfection efficiency I ever saw with ESCs. Lipofectamin 3000 didn't give me these results (used on mouse ESCs).

--------Marina Pryzhkova, Ph.D., JHU

I tried the plasmid/siRNA co-transfection using the LipoJet sample in EAhy926 cell which is a HUVEC cell line. The results are pretty good, at least for the transfection efficiency. For the plasmid transfection, 48 hours later, around 60% cell are transduced. For siRNA, the efficiency is almost 100%. We are ordering more LipoJet reagent to use from now.

-----Dr. Pan Kong, University of Southern California

我们在293T单元格上使用Lipojet的效率> 90％，而X-三分法9的效率为80％。肯定会订购更多...

-----Dr. Nuo Yang from Roswell Park Cancer Institute

Here are the results from our preliminary experiments with LipoJet. We are very satisfied with its efficiency. Unfortunately, we were out of Fugene 6/HD and were unable to test them side by side, but based on previous experience, we do believe that your product worked with better efficiency.

------Dr. Alison McKelvey from University of Pittsburgh

I am happy to say that I had great success with your transfection reagents on OKF6/TERT2 human keratinocytes. I will put together the data once I have completed my analysis. I really like the LipoJet. My cell of interest did not do very well in the GenMute although my control cells did. We want to go ahead with ordering ASAP. I also have a coupon for 10% for a PO order.

-----Dr. Michelle Simpson-Abelson from UPMC

We did indeed test them side by side with our homemade CaPhos. The results are great, LipoJet and CalFectin both did extremely well. LipoJet being the better of the two. I will send you the results when I get them, I am currently out of the lab but we will be ordering more for certain. Thank you for sending those samples.

------Dr. Ahmed Hassib, Cornell University

For the LipoJet, I only compared it with Lipo LTX in Hela cells. The LipoJet gave a much higher efficiency (~35% better than Lipo LTX) 24 hours after transfection. We will order some LipoJet.

----- USC的Beta测试仪