Pre-made recombinant lentivirus; LV-CaMKII-GCaMP6f; CaMKII-GCaMP6f Lentivirus
Product Category:Pre-made recombinant lentivirus; LV-CaMKII-GCaMP6f; CaMKII-GCaMP6fLentivirus
Description:
GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase.When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms.
GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast - GCamP6f, slow - GCaMP6f and medium - GCaMP6m).
LV-CaMKII-GCaMP6fis pre-made lentivirus which expressesGCaMP6funder CaMKII promoter for neuronal exclusive expression. Ready to use format.
Titer:> 1x109TU/mL
Selection Antibiotic:None
Purity*:In vivo grade and OK for both in vitro tissue culture infection and in vivo injection
Size:2 x 25µL (Std Pack)
Storage Buffer:PBS
Infection Protocol:
Transfer Vector Map:
*Concentrated viaPEGprecipitation followed by ultra-centrifugation at 25000 rpm
