LipoD293� In Vitro DNA Transfection Reagent

Description
By utilizing our innovative and proprietary lipid conjugation technology, LipoD293� (Ver. II) is specially designed and formulated by adding proprietary enhancers for transfecting HEK293 cells and other mammalian cells (Figure 1). As a 2nd generation liposome based DNA transfection reagent, LipoD293� (Ver. II) offers extremely high transfection efficiencies for HEK293 related cells as well as many mammalian cells with less cytotoxicity. LipoD293� reagent (Ver. II), upgraded from its previous version with refined chemistry, is 3~4 times more efficient in gene delivery. LipoD293� reagent (Ver. II), 1.0 ml, is sufficient for ~666 transfections in 24 well plates or ~333 transfections in 6 well plates.


Figure 1.显示Lipod293的动画片（VER。II）增强的基因递送

Features
- 难以转化的单元格的最佳选择
- Exceptional high titers of virus production
- Equally good for very long DNAs (up to 180 kb)
- 同样适用于悬架293个单元格（例如293F，293H等）
- High levels of recombinant protein production
- 血清和抗生素的存在提高了293个细胞的效率
- 单DNA转染和多DNA共转染的特殊效率
- Very affordable

储存情况
Store at 4 �C. If stored properly, the product is stable for 12 months or longer.

Comparisons of Transfection Efficiency of LipoD293� DNA In Vitro Transfection Reagent (Ver. II) with Brand Name Products

比较转染效率LipoD293�reagent (Ver. II) vs. lipofectamine 2000 (L2K) and Fugene HD on HepG2 cells.
右面板：Comparison of transfection efficiency of LipoD293 (Ver. II) with Lipofecatmine 2000 (L2K), and Fugene HD on HepG2 cells. GFP DNA (pEGFP-N3) was transfected with different transfection reagents per manufacturer's protocols to HepG2 cell (cultured on Collagen pretreated dishes). GFP positive cell (%) and fluorescence intensity were detected by passing through FACS 48 hours post transfection
左图：血清和抗生素的存在增强了LIPOD293（VER。II）对HEPG2细胞的效率。HEPG2细胞（在经过胶原蛋白治疗的菜肴上生长）用三种不同的疾病转染-------无血清和抗生素，存在10％血清和抗生素，然后在转染后去除5小时，并存在10％血清和抗生素，没有转染后5小时清除。


比较转染效率LipoD293�reagent (Ver. II) vs. lipofectamine 2000 (L2K), TransIT and Fugene 6 on CHO cells.
右面板：LiPOD293（VER。II）与Lipofecatmine 2000（L2K），Transit和Fugene 6的转染效率的比较。根据制造商的方案，用不同的DNA转染试剂转染编码Renilla荧光素酶（PHRL-CMV）和GFP（PEGFP-N3）的DNA。转染后24小时24小时，用Renilla分析系统和尼康Eclipse荧光微拷贝检测到Renilla荧光素酶活性和GFP荧光。
Left Panel:比较LipoD293的价格（$/1.0毫升小瓶）与Lipofecatmine 2000（L2K），Transit和Fugene 6的比较。所有价格均从制造商的网站收集。


A comparison of transfection efficiency of LipoD293� reagent with lipofectamine 2000 (L2K) on a hard-to-transfect cell, primary rat aortic smooth muscle cells.The rat aortic smooth muscle cells were prepared and transfected with pEGFP-N3 by LipoD293� reagent (left panel) and Lipofecatmine 2000 (L2K, right panel) respectively per manufacturers' protocols. The transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon Eclipse 2000 microscopy 24 hours post transfection. The above pictures were kindly provided by Dr. Nickolai Dulin of Section of Pulmonary and Critical Care, University of Chicago.


A comparison of transfection efficiency of LipoD293� reagent with Fugene HD on hard-to-transfect cell, LNCap cells.The LNCap cells were grown as ATCC recommended procedures and co-transfected with pBabe-hygro-SSeCKs (1.5ug) and pEGFP-N3 (0.5ug) per well (6 well plate) LipoD293� reagent (left panel) and Fugene HD (right panel) respectively per manufacturers' protocols. The transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon Eclipse 2000 microscopy 24 hours post transfection. The above pictures were kindly provided by Dr. Lyn Gao of Roswell Park Cancer Institute.


两个例子显示了LIPOD293的特殊效率。在存在血清/抗生素的情况下，分别用PEGFP-N3和PSV - ？？ - 半乳糖苷酶DNAS转染95％汇合的HEPG2和SAOS-2细胞。Zeiss 510共聚焦显微镜和？ - 半乳糖苷酶染色试剂盒转染后48小时检查效率。



A comparison of transfection efficiency of LipoD293� reagent with 293fectin on HEK293 cells.HEK293 cells transfected with pEGFP-C1 plasmid using LipoD293� In Vitro DNA Transfection Reagent (Ver. II) (upper panel) and the most popular brand product 293fectin� of Invitrogen (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection.


A comparison of LipoD293� reagent vs. 293fectin, Xfect and Fugene 6 transfection reagents on protein production with suspension 293F cells. 30 mL of使用LIPOD293。在标准培养基中培养的293F细胞用PEGFP-6XHIS质粒转染了体外DNA转染试剂（VER。II）（20 ug质粒DNA），293fectin（30 ug质粒DNA），Xfect（30 UG质粒DNA）（30 UG质粒DNA）（30 UG质粒DNA）根据制造商的标准转染方案，Fugene 6（30 ug质粒DNA）。转染后48小时可视化GFP荧光（左图），a lipod293，b，用于293fectin，c for xfect和f for fugene 6. 6 xhis标记的GFP蛋白，然后纯化VI。a Ni-NTAaffinitY列。在SDS-PAGE上解析5 ul的第一个洗脱部分，然后在coomassie亮蓝色染色（右上图E）上进行coomassie亮蓝色（右上图E），泳道1用于Lipod293，plotein Marker的泳道2，Xfect 3泳道，Xfect 4，泳道4，用于293fectin和5Fugene 6.通过光谱仪（右下图F）定量蛋白质产率。



A comparison of LipoD293� (Ver. II) and Lipofecatmine LTX (LTX) on generation of Lentivirus (LV).三个cDNA与Lipod293。（Ver。II）和Lipofectamine LTX（LTX）共转染到293ft细胞中。GFP载体PHR-SIN-CPPT-CMVEWP用于确定LV的滴度。将每个孔的1x105 293F细胞铺在24井板上，然后分别添加不同量的载体上清液，1个微晶（上图）和10个微层（下图）。5天后，细胞与FACS通过。右上角的数字表示转导细胞的百分比。用LiPOD293。和L2K生成的LV的滴度分别量化为8x10^6和3x10^6 tu/ml

Technical Information & Datasheet
-LipoD293� (Ver. II) Reagent General Protocol & Data Sheet
-LipoD293� (Ver. II) Reagent Short Protocol
-LipoD293� (Ver. II) Reagent Protocol for Transfecting Suspension 293 and CHO Cells
-Lipod293。（Ver。II）慢病毒的试剂
-Lipod293。（VER。II）RAAV生产的试剂
-技术说明和转染技巧


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Testimonials
I am absolutely thrilled with the LipoD293 transfection reagent! I compared LipoD293 to Lipofectamine for transfection of plasmids to generate viral pseudoparticles and WOW! I recovered 4 times more virus using the LipoD293 reagent than Lipofectamine!! This is wonderful news for me and my mentor as it means that I don't have to spend so much time (and resources) on transfecting 293T cells to recover virus for in vitro experiments. My mentor was also very happy that LipoD293 costs much less than Lipofectamine!! We have already ordered 5 mls of LipoD293 and I have now convinced my lab mates to switch to LipoD293 since I got such good results with it. Thanks for making such a great product!
-------- Christy Lavine, Ph.D., Harvard University

I wanted to update you on the free sample of LipoD293 that you sent to us. I recently transfected some Plat-E's side-by-side with Lipofectamine 2000, and your product out-performed it!!! We are also validating it with HeLa cells, but otherwise we are VERY happy with what we have seen thus far! Thank you for sending us the free sample, it definately made the SALE for us!
--------辛辛那提大学凯瑟琳·加洛（Catherine Gallo）博士

现在，我们已经在293FT细胞上尝试了LIPOD293 DNA的体外转染试剂。在第一个烧瓶中，我们使用了Virapower盒插入物中提供的Invitrogen（Virapower，Pbabe-GFP质粒，以及Lipofectamine）的方案。在第二瓶中，我们使用了Lipod293（30 UL/6ML介质），其含量与第一个烧瓶相同。结果令人惊叹：lipod293的转化剂是Lipofectamine 2000的两倍...
--------韦恩州立大学的Beta测试师