Genmute�.siRNA Transfection Reagent

Description:
Genmute�.Reagent is a novel biodegradable non-liposomal siRNA/miRNA & DNA delivery tool. With our proprietarypH Dependent Conformational Change (PDCC）技术，通过将预筛分的疏水基团加入其侧链，制作，可生物降解转染试剂。Genmute�.Reagenta versatile and most powerful gene delivery tool in the market.Genmute�.Reagenthave been validated to effectively and reproducibly transfect single siRNA/miRNA or co-transfect DNA/siRNA or DNA/miRNA to variety of mammalian cells.

Size & content:
- 基因酮试剂，1.0ml，足以基于转染5.0pmol siRNA或MiRNA模拟物在24孔板中进行的〜2,000反应。
- 基因骨转染缓冲液（5倍），配制成最大转染效率，8.0ml制备40 mL的工作溶液。

应用程序:
- Single siRNA transfection or DNA/siRNA co-transfection to variety of mammalian cells.
- 单一miRNA，miRNA模拟物或抑制剂转染或DNA / miRNA或DNA / miRNA模拟与哺乳动物细胞各种的共转染。

Storage:
Store at 4 �C forGenmute�.Reagent and RT forGenmute�.转染缓冲液（5x）. If stored properly, the product is stable for 12 months or longer.

Advantages:
- Excellent silencing at low concentrations with only 1.0 nM siRNA / miRNA mimics
- Minimizing non-specific and off target effects in the cell
- One tube reaction
- Best for broad mammalian cells
- Very low cytotoxicity
- 非常实惠

与品牌产品的Genmute®siRNA和DNA转染试剂沉默疗效的比较

基因骨转染试剂（上图）与DHArmafect4siRNA转染试剂（中间板）的敲低疗效比较and Lipofectamine� 2000 (lower panel) on HEK293 cells.靶向从0.5-20nm的不同最终浓度的renilla荧光素酶的siRNA与每种制造商方案的上述三次转染试剂的Renilla Luciferase基因（每孔0.5μgPrl-CMV DNA）共转染到生长的HEK293细胞中24孔板。Renilla luciferase activity was determined 24h after post co-transfection with renilla luciferase determination system (Promega). The luminescence was measured from 5.0 �l of lysate during 10s integration with a luminometer (Beckman Coulter LD 400). Luciferase activity wasexpressed as light units integrated over 10s (RLU) and normalized per mg of cell protein by using the BCA assay. The errors bars represent standard deviation derived from triplicate experiments. Luciferase-silencing efficiency was calculated relative to untreated cells.尽管Genmute1和Dharmafect = 4试剂从1.0nm的Renilla Luciferase siRNA，Li递送了显着的基因沉默pofectamine� 2000 gave good knockdown only after 30 nM (data not shown).


在HEK293细胞中优异地沉默于内源性表达KIF11（也称为EG5），其中GENMU0.5μLTe�试剂和5.0pmol Eg5每孔24孔板的SiRNA。KIF11 (also known as Eg5) encodesa motor protein that belongs to thekinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. GenMute� reagent effectively delivers Eg5 siRNA (final 10 nM, right panel) to HEK293 cells at only 0.5 �l per well of 24-well plate vs. negative control (final 10 nM. left panel). Compared with negative control (left panel), phenotype of "rounded-up" 293 cells were visualized 24 hours post transfection (right panel) with a Nikon microscope.


Genmute�.Transfection Reagent knocked down endogenous lamin A/C gene expression in Hela cells.A siRNA targeting lamin A/C gene (right panel) and a sham siRNA (left panel) were introduced into Hela cells (final 1.0 nM) byGenmute�.Transfection Reagent. Lamin A/C gene silencing was monitored 24h post transfection by immunofluorescence. Lamin A/C was probed with a mouse monoclonal lamin A/C specific antibody followed by addition of FITC-conjugated anti-mouse antibodies. Quantitative analysis showed thatlamin siRNA at 1.0 nM delivered by GenMute� Transfection Reagent knocked down 94% endogenously expressed lamin A/C in Hela cells.

Data Sheet & Protocol
-A Protocol for siRNA Transfection and DNA/siRNA Co-Tranafection
-A Protocol for miRNA Mimics Transfection and DNA/miRNA Co-Transfection
-A Protocol for DNA Transfection to Mammalian Cells

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